WebUsed in wide variety of tissue culture applications. BioWhittaker™ Hank's Balanced Salt Solutions (1X) is used to maintain a physiological pH for cells maintained in non-CO2 conditions, diluting cells before counting, washing cells before dissociating and transporting cells or tissues. WebUsed to remove cells from the culture vessel surface into suspension Shop Lonza™ BioWhittaker™ Trypsin Reagent at Fishersci.fi Lonza™ BioWhittaker™ Trypsin Reagent Trypsin in HBSS w/o Calcium and Magnesium; 100mL Lonza™ BioWhittaker™ Trypsin Reagent Fisher Scientific
Lonza™ BioWhittaker™ Trypsin Reagent Trypsin in HBSS w/o …
WebCell Culture Reagent Lonza™ BioWhittaker™ Hanks' Balanced Salt Solution (HBSS) 1X / pH 7.14 to 7.46 500 mL Fisher Scientific BW10547F Fisher Scientific BW10547F - … WebNormal human skeletal muscle cells (Clonetics™) and the recommended growth medium are available from BioWhittaker, Inc. (Walkersville, MD). 4. ... Add 0.2% (w/v) collagenase type XI in HBSS and dissociate the muscle tissue by enzymatic digestion at 37°C for 1 h, with continuous gentle rocking of the conical tube. ... buckley\u0027s in queen anne seattle wa
BioWhittaker Classical Media for Consistent Cell …
Web1. Hank’s balanced salt solution (HBSS) (with Ca 2+/Mg ): 0.185 g CaCl 2 · H 2O, 0.4 g KCl, 0.06 g KH 2PO 4, 0.1 g MgSO 4 · 7 H 2O, 8 g NaCl, 0.35 g NaHCO 3, 0.048 g Na 2HPO 4, and 1 g D-glucose per liter water. The solution must be sterile filtered, when not obtained in ready-to-use form. We obtain the solution from WebOct 1, 2024 · Hanks' balanced salt solution (HBSS) (BioWhittaker Lonza, Basel, Switzerland) and Iscove's Modified Dulbecco's Medium (IMDM) (BioWhittaker Lonza) were supplemented with 10% fetal bovine serum (FBS) (Hy-Clone Fetal bovine serum defined; Thermo Scientific, Waltham, MA, USA) and 1% penicillin/streptomycin if not otherwise … WebHBSS is a balanced salt solution to be used in a wide variety of tissue culture applications. Component (mg/L) 04-315 08-003 10-508 10-527 10-543 10-547 CaCl 2•2H 2O 186 186 KCl 400 400 400 400 400 400 KH 2PO 4 60 60 60 60 60 60 MgSO 4•7H 2O 200 200 NaCl 8000 8000 8000 8000 8000 8000 ... credit union for students